U of M: Department of Genetics, Cell Biology and Development

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	Perry Hackett, Ph.D. Professor
	Areas of Research: Research Techniques: Research Interests: Selected Publications: Related Links

Mailing Address: University of Minnesota Department of Genetics, Cell Biology, and Development 6-160 Jackson 321 Church St. SE Minneapolis, MN 55455 USA		Education: Ph.D., University of Colorado, 1974
Office: 5-108 MCB P: 612-624-6736 F: 612-626-6140 Email: hacke004@umn.edu	Lab: 5-206 MCB P: 612-624-4206

Areas of Research Strength:

Development of transgenic vectors for vertebrates.
Developing non-viral vectors for human gene therapy.
Analysis of gene expression in transgenic animals.
Analysis of integration sites of vectors in transgenic animals.

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Research Techniques:

Nearly all methods involving recombinant DNA technology.
Methods for delivery of transgenic vectors to chromosomes in vertebrates.
Methods for analyzing gene expression in transgenic animals.
Methods for identifying integration sites of vectors in transgenic animals.

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Research Interests:

The Hackett lab's main area of interest is using transposons as vectors
for gene therapy as well as tagging and mapping genes in vertebrate
chromosomes. For this, we use the Sleeping Beauty Transposon System,
so called because we awakened the transposons from an evolutionary
sleep of more than 10 million years. The Sleeping Beauty system appears
to be the most efficacious method for inserting precise, single expression
cassettes into human chromosomes without using viruses. The lab works
very closely with other labs in the Beckman Center for Transposon Research
including the Ekker, McIvor and Largaespada labs. The lab is also developing
transposon tools for studying gene expression in vertebrates and analyzing
the roles of specific genes in vertebrate development.

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Selected Publications:

Hackett, C.S., A.M. Geurts and P.B. Hackett (2007) Predicting DNA vector insertion sites: Implications for functional genomics and gene therapy. Genome Biol. (in press)

Aronovich, E.L., J.B. Bell, L.R. Belur, R. Gunther, B. Koniar, D.C. Erickson, P.A. Schachern, R.S. McIvor, C.B. Whitley and P.B. Hackett (2007). Sleeping Beauty transposon-mediated gene therapy of murine mucopolysaccharidosis. J. Gene Med. (in press).
Online in advance of print: http://www3.interscience.wiley.com/cgi-bin/fulltext/114206158/HTMLSTART

Hackett, P.B. (2007). Integrating DNA vectors for gene therapy. Mol. Ther. 15: 10-12.

Balciunas, D., K.J. Wagensteen, A. C. Wilber, J.B. Bell, A.M. Geurts, S. Sivasubbu, X. Wang, P.B. Hackett, D.A. Largaespada, R.S. McIvor, and S.C. Ekker. (2006). Harnessing an efficient large cargo-capacity transposon for vertebrate gene transfer applications. PLoS Genetics.

Wilber, A., J.L. Frandsen, A.M. Geurts, D.A. Largaespada, P.B. Hackett, and R.S. McIvor (2006). RNA as a source of transposase for Sleeping Beauty-mediated gene insertion and expression in somatic cells and tissues. Mol Ther. 13: 625-630.

Geurts*, A.M., C.S. Hackett*, J.B. Bell, T.L. Bergemann, C.M. Carlson, L.S. Collier, D.A. Largaespada and P.B. Hackett (2006). Structure-based prediction of insertion-site preferences of transposons into chromatin. .Nucl. Acids Res. 34: 2803-2811.

Score, P.R., L.R. Belur, J.L. Frandsen, J. Geurts, T. Yamaguchi, N.V. Somia, P.B. Hackett, D.A. Largaespada and R.S. McIvor (2006). Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP-Cre recombinase system to distinguish transposition-specific expression. Mol. Therap. 13:617-624.

Geurts, A.M., A.C. Wilber, C.M. Carlson, P.D. Lobitz, K.J. Clark, P.B. Hackett, R.S. McIvor, D.A. Largaespada (2006). Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon. BMC Biotech 6(1): 30.85.

Liu, G., A.M. Geurts, K. Yae, A.R. Srinivassan, S.C. Fahrenkrug, W.K. Olson J. Takeda, K. Horie and P.B. Hackett (2005). Target-site preference for Sleeping Beauty transposons. J. Mol. Biol. 346: 161-173.

Ohlfest, J.R., J.L. Frandsen, S. Fritz, P.D. Lobitz, S.G. Perkinson, K.J. Clark, N.S. Key, R.. McIvor, P.B. Hackett and D.A. Largaespada (2005). Phenotypic correction and long-term Factor VIII expression in hemophilic mice by immunotolerization and nonviral gene transfer using the Sleeping Beauty transposon System. Blood 105: 2691-2698.

REVIEWS:

Hackett, P.B. and K.C. Clark (2007). Functional Genomics. In Aquaculture Genome Technologies (Z. Liu, ed.). Blackwell Publishers Inc. Chapter 24, pp. 429-462.

Essner, J.J., R.S. McIvor and P.B. Hackett (2005). Awakening of gene therapy with Sleeping Beauty transposons. Curr. Opin. Pharmacol. 5(5): 513-519.

Hackett, P.B., S.C. Ekker, D.A. Largaespada and R.S. McIvor (2004). Sleeping Beauty transposon-mediated gene therapy for prolonged expression. In Huang, L., E. Wagner & M-C. Hung, eds. Non-Viral Vectors for Gene Therapy, 2^nd Edition, Adv. Genet. 54: 187-229.

Hackett, P.B., K.J. Clark, S.E. Ekker and J.J. Essner (2004). REVIEW: Applications of Transposable Elements in Fish for Transgenesis and Functional Genomics. Fish Development and Genetics (Z. Gong and V. Korzh, eds.) Chapter 16, 532-580.

To view these and other publications visit http://www.ncbi.nlm.nih.gov/PubMed
search menu should say PubMed
type Hackett PB in the avaliable line

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Related Links:

http://beckmancenter.ahc.umn.edu/
http://discoverygenomics.net
http://biosci.cbs.umn.edu/labs/perry/welcome.htm

SBST Bibliography

ProTIS Instructions
ProTIS.pl

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