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David W. Hamilton, Ph. D.

Professor

Mailing Address:
University of Minnesota
Department of Genetics, Cell Biology, and Development
6-160 Jackson
Minneapolis, MN 55455
USA


Office:
4-135A Jackson Hall
P: 612-624-6606
F:612-624-8118

Email:
dwh@umn.edu
 Lab:
4-242 BSBE
P: 612-624-0674

Education:
BA, 1957, Harvard College, Cambridge, MA;
MA, 1960, University of Kansas, Lawrence, KS;
Ph.D., 1964, Cambridge University, England;
Research Fellow, 1963-1965, Department of Anatomy, Harvard Medical School, Boston, MA.


Honors:
Rockefeller Foundation Special Fellow in Reproductive Biology, Cambridge University, England, 1970-1971; Lawrence J. Henderson Associate Professor of Health Sciences and Technology, Harvard University/Massachusetts Institute of Technology, 1974-1977; Vice President, American Society of Andrology, 1990-1991; President, American Society of Andrology, 1991-1992; Co-Editor-in-Chief Journal of Andrology, 1998-2003; Distinguished Service Award, American Society for Andrology, 1999; President's Outstanding Service Award, University of Minnesota, 2000.


Areas of Research Strength:

Signaling cascades involved in sperm capacitation and the acrosome reaction;
Molecular Aspects of Sperm Maturation;
Fertilization.



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Research Techniques:

Molecular techniques (recombinant DNA, PCR, Southerns,
northerns and all techniques necessary to clone and mutate genes),
monoclonal antibody production, immunocytochemistry, light and
electron microscopy, image analysis, calcium imaging, protein
purification and analysis.  Techniques are acquired to address
the specific biological question.


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Research Interests:

During the past few years the work in the Hamilton lab has focused in three areas
of reproduction:

- signaling events that occur in sperm during capacitation in the female
reproductive tract (an early process that prepares sperm to bind to the egg),
- the calcium-dependent acrosome reaction (an exocytotic event that occurs
after sperm have bound to the egg), and
- fertilization (during which the sperm and egg fuse).

The lab will continue to work in these areas in the future, particularly focusing
on regulation of fertility by sperm-associated membrane proteins acquired
during maturation.


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Selected Publications:

Roberts KP, Ensrud KM, Wooters JL, Nolan MA, Johnston DS, Hamilton DW. 2006 Epididymal secreted protein Crisp-1 and sperm function. Mol Cell Endocrinol. 250(1-2):122-7.

Roberts KP, Wamstad JA, Ensrud, KA, Hamilton DW 2003 Inhibition of Capacitation-Associated Tyrosine Phophorylation Signaling in Rat Sperm by Epididymal Protein Crisp-1. Biol Reprod 69:572-581

Roberts KP, Ensrud, KM, Hamilton, DW 2002 A Comparative Analysis of Expression and Processing of the Rat Epididymal Fluid and Sperm-Bound Forms of Proteins D and E. Biol. Reprod. 67:525-533.

Roberts KP, Hoffman LB, Ensrud KM, Hamilton DW 2001 Expression of Crisp-1 mRNA Splice Variants in the Rat Epididymis, and Comparative Analysis of the Rat and Mouse Crisp-1 Gene Regulatory Regions. J Androl. 22:157-163.

Klemme, L.M., K. Roberts, L. Hoffman, K. Ensrud, J. Siiteri ,and D.W. Hamilton 1999 Cloning and characterization of the rat Crisp-1 gene. Gene 240, 279-288.

Xu, W. K. Ensrud and D.W. Hamilton 1997 The 26 kD protein recognized on rat cauda epididymal sperm by monoclonal antibody 4E9 has internal peptide sequence identity with the secreted form of rat epididymal protein E. Mol. Reprod. Dev. 46:377-382.

Xu, W and DWHamilton 1996 Identification of the rat epididymis-secreted 4E9 antigen as protein E. Further biochemical characterization of highly homologous epididymal secretory proteins D & E. Mol. Reprod. Dev. 43:347-357.

Raab, LS, KL Polakoski, LW Hancock and DW Hamilton 1995 Characterization of the heat shock protein P70 in rat spermatogenic cells. Mol. Reprod. Dev. 40:186-195.

Siiteri, JE, K Ensrud, A Moore and DW Hamilton 1995 Identification of osteopontin (OPN) mRNA and protein in rat testis and epididymis, and on sperm. Mol. Reprod. Dev. 40:16-28.

Cesario, MM, K Ensrud, DW Hamilton, JR Bartles 1995 Biogenesis of the posterior-tail plasma membrane domain of the mammalian spermatozoon: Targeting and lateral redistribution of the posterior-tail domain-specific transmembrane protein CE9 during spermiogenesis. Develop. Biol.169:473-486.

Raab, LS, DW Hamilton and LW Hancock 1994 Proacrosin gene expression in rat spermatogenic cells. J. Androl. 15(3): 244-249.

Moore, A, K Ensrud, T White, C Frethem and DW Hamilton 1994 Rat epididymis-specific sperm maturation antigens. I. Evidence that the 26 kD 4E9 antigen found on rat cauda epididymal sperm is derived from a protein secreted by the epididymis. Mol. Reprod. Dev. 37:181-194.



To view these and other publications visit http://www.ncbi.nlm.nih.gov/PubMed
search menu should say PubMed
type Hamilton DW in the available line

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Distribution of lipid rafts in rat sperm before (A) and after (B) in vitro capacitation

 
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 Last modified on Monday, July 24, 2006